Anti-PML antibody, Mouse monoclonal
SIGMA/P6746 - clone PML-97, purified from hybridoma cell culture
Synonym: Anti-Promyelocytic Leukemia
MDL Number: MFCD06412254
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 10 μg/mL Monoclonal Anti-PML Clone: PML-97 (Cat. No. P6746). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HEK-293T cells overexpressing human PML3 was separated on SDS-PAGE and probed with Monoclonal Anti-PML Clone: PML-97 (Cat. No. P6746). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative control: No 1st Antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PML-97, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | hamster, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using 293T cells expressing recombinant PML3 | |
| UniProt accession no. | P29590  |
| Application: | Anti-PML antibody, Mouse monoclonal may be used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunocytochemistry • indirect immunofluorescence |
| Biochem/physiol Actions: | A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport. |
| Biochem/physiol Actions: | Monoclonal Anti-PML recognizes human, mouse, and hamster PML (about 90 kDa) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm. |
| General description: | The PML gene is located on the human chromosome at 15q24.1. |
| Immunogen: | recombinant full-length human PML (promyelocytic leukemia) protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |