Anti-p53 antibody, Mouse monoclonal
SIGMA/P6749 - clone PAb1801, purified from hybridoma cell culture
Synonym: Anti-p53 antibody, Mouse monoclonal; Anti-TRP53; Anti-
MDL Number: MFCD00164709
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.06 μg/mL Monoclonal Anti-p53 Clone: PAb1801 (Cat. No. P6749). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. A431 2. HEK-293T 3. RAJI
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PAb1801, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~53 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.03-0.06 μg/mL using total cell extract of A431 cells | |
| UniProt accession no. | P04637  |
| Application: | Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting. |
| Biochem/physiol Actions: | Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-p53 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma PAb1801 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. Anti-p53 antibody, Mouse monoclonal recognizes human p53. p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |