PIPES
SIGMA/P6757 - ≥99% (titration)
Synonym: 1,4-
CAS Number: 5625-37-6
Empirical Formula (Hill Notation): C8H18N2O6S2
Molecular Weight: 302.37
EC Number: 227-057-6
MDL Number: MFCD00006159
Linear Formula: C8H18N2O6S2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6757-100G
This image depicts SKU: P6757-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6757-500G
This image depicts SKU: P6757-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: P6757-1KG
This image depicts SKU: P6757-1KG
| application(s) | diagnostic assay manufacturing |
| assay | ≥99% (titration) |
| form | crystalline powder |
| InChI | 1S/C8H18N2O6S2/c11-17(12, |
| InChI key | IHPYMWDTONKSCO-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| pKa (25 °C) | 6.8 |
| Quality Level | 400  |
| SMILES string | OS(=O)(=O)CCN1CCN(CC1)CCS |
| solubility | 1 M NaOH: 20 + 80 mL g, clear, colorless |
| useful pH range | 6.1-7.5 |
| Application: | Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative., Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer. |
| General description: | PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work. |
| Other Notes: | Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851 ) as well as several different salts for convenience in buffer preparation, and PIPES BioXtra (Product No. P8203 ) which is tested for the presence of trace metals. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 25 kg in poly drum |
| Packaging: | 25, 100, 500 g in poly bottle |
| Preparation Note: | Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (titration) |
| mp | >300 °C (lit.) |
| UNSPSC | 12161700 |