Peroxidase from horseradish
SIGMA/P6782 - Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol)
Synonym: Donor:hydrogen-
CAS Number: 9003-99-0
EC Number: 232-668-6
MDL Number: MFCD00071339
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6782-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6782-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6782-25MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P6782-50MG
| application(s) | diagnostic assay manufacturing |
| form | essentially salt-free, lyophilized powder |
| InChI | 1S/H2O3/c1-3-2/h1-2H |
| InChI key | JSPLKZUTYZBBKA-UHFFFAOYSA |
| mol wt | ~44 kDa |
| Quality Level | 300  |
| SMILES string | [O+H2]O[O-] |
| solubility | 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0) |
| H2O: soluble | |
| specific activity | ≥250 units/mg solid (using pyrogallol) |
| 950-2000 units/mg solid (using ABTS) | |
| storage temp. | 2-8°C |
| type | Type VI-A |
| Analysis Note: | The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. |
| Analysis Note: | This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid |
| Application: | Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6782 is type VI-A and it is essentially a salt-free, lyophilized powder. It is used to quantify cholesterol and cholesterol esters and to study in vitro lipid deposition on hydrogel and silicone hydrogel contact lenses. |
| Application: | The enzyme from Sigma has been used in the development of rapid and sensitive galactose oxidase-peroxidase biosensor for galactose detection with prolonged stability. It has been used as a component of a media (MRS agar) plate containing 3,3′,5,5′-tetramethylbenz |
| Biochem/physiol Actions: | HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, as well as Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions are found to inhibit the enzyme activity. |
| Biochem/physiol Actions: | When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. |
| General description: | Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. |
| Other Notes: | One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0 |
| Other Notes: | One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C. |
| Other Notes: | Similar to P8375 |
| Other Notes: | View more information on peroxidase at www.sigma-aldrich.com/enz. |
| Packaging: | 10, 25 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Packaging: | 50, 100 mg in glass bottle |
| Packaging: | Packaged in mg solid |
| Preparation Note: | Solutions of this product at 1 mg/mL in 0.1 M phosphate buffer (pH 6.0) remain active for at least two weeks at room temperature. The solution retains activity after 5 freeze-thaw cycles. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥250 units/mg solid (using pyrogallol); specific activity: 950-2000 units/mg solid (using ABTS) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |