Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse
SIGMA/P6834 - clone PP-67, ascites fluid
Synonym: Anti-Ki-67
MDL Number: MFCD00803547
Product Type: Chemical
Immunohistochemistry Staining of microwave-treated formalin-fixed, paraffin-embedded human tonsil sections with 1:800 Monoclonal Anti-Proliferating Cell Protein Ki-67, Clone: PP-67 (Cat. No. P6834) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence SK-MEL-28 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:200 Monoclonal Anti-Proliferating cell protein Ki-67, Clone: PP-67 (Cat. No. P6834). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PP-67, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using microwave-treated human tonsil sections | |
| microarray: suitable | |
| UniProt accession no. | P46013  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry. Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse has also been used for immunohistochemistry. |
| Biochem/physiol Actions: | Marker of proliferation Ki-67 (MKI67), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker. |
| Biochem/physiol Actions: | Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker. Ki-67 is considered as poor prognostic factors of adenoid cystic carcinoma. |
| Biochem/physiol Actions: | Reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Marker of proliferation Ki-67 (MKI67) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases of the cell cycle. The gene encoding this protein is localized on human chromosome 10. |
| General description: | Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases (G1, S, G2, and mitosis) of the cell cycle. The gene encoding this protein is localized on human chromosome 10. |
| Immunogen: | HeLa cell nuclear preparation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |