Anti-p53 antibody, Mouse monoclonal
SIGMA/P6874 - clone DO-1, purified from hybridoma cell culture
Synonym: Anti-BCC7; Anti-BMFS5; Anti-LFS1; Anti-P53; Anti-TRP53
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.4 μg/mL Monoclonal Anti-p53 Clone: DO-1 (Cat. No. P6874). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. A431 2. HEK-293T 3. RAJI
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DO-1, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~53 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells | |
| UniProt accession no. | P04637  |
| Application: | Anti-p53 antibody, Mouse monoclonal has been used in: • semi-dry western blotting • immunoblotting • immunofluorescence • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • immunohistochemistry • immunoprecipitation • flow cytometry • chromatin immunoprecipitation (ChIP) assay |
| Biochem/physiol Actions: | Mouse anti-(p53) antibody reacts specifically with (p53) if human. |
| Biochem/physiol Actions: | The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues. |
| Immunogen: | recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |