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Phospho(enol)pyruvic acid trisodium salt hydrate

SIGMA/P7002 - ≥97% (enzymatic)

Synonym: 2-(Phosphonooxy)-2-propenoic acid trisodium salt; PEP

CAS Number: 5541-93-5 (anhydrous)
Empirical Formula (Hill Notation): C3H2Na3O6P · xH2O
Molecular Weight: 233.99 (anhydrous basis)
MDL Number: MFCD00150734
Linear Formula: C3H2O6PNa3 · x H2O
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-P7002-100MG 100 mg
$99.90
1/EA
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45-P7002-250MG 250 mg
$165.00
1/EA
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45-P7002-500MG 500 mg
$334.00
1/EA
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45-P7002-1G 1 g
$543.00
1/EA
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45-P7002-5G 5 g
$2000.00
1/EA
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45-P7002-25G 25 g
$7500.00
1/EA
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assay ≥97% (enzymatic)
form powder
InChI 1S/C3H5O6P.Na.H/c1-2(3(4)5)9-10(6,7)8;;/h1H2,(H,4,5)(H2,6,7,8);;
InChI key KZPUEZVJSLLABH-UHFFFAOYSA-N
Quality Level 200 
SMILES string [Na].OC(=O)C(=C)OP(O)(O)=O
solubility water: 100 mg/mL, clear to slightly hazy, colorless to very faintly yellow
storage temp. −20°C
Application: Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:
• several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay
• in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor α (PDGFRA) kinetic parameters
• as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement

Biochem/physiol Actions: Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.
Biochem/physiol Actions: Phosphoenolpyruvic acid plays a major role in the initial step of glycolysis by acting as an inhibitor of hexokinase, phosphoglucose isomerase, phosphofructokinase and aldolase.
Purity ≥97% (enzymatic)
Storage Temp. −20°C
UNSPSC 12352204

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