Pyruvate Dehydrogenase from porcine heart
SIGMA/P7032 - buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
Synonym: PDH; Pig heart pyruvate dehydrogenase
CAS Number: 9014-20-4
MDL Number: MFCD00132145
Product Type: Chemical
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| concentration | ≥5.0 mg/mL |
| foreign activity | α-ketoglutarate dehydrogenase ≤15% |
| form | buffered aqueous glycerol solution |
| impurities | polyethylene glycol, trace |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | 0.1-1.0 units/mg protein (Lowry) |
| storage temp. | −20°C |
| Application: | Pyruvate dehydrogenase contributes to linking the glycolysis metabolic pathway to the citric acid cycle and releases energy via NADH. It is used to study how aerobic training effects pyruvate dehydrogenase . It is also used to study the interaction between pyruvate dehydrogenase kinase and the pyruvate dehydrogenase complex as well as the function and regulation of the pyruvate dehydrogenase complex. Product P7032 is provided in a buffered aqueous glycerol solution and is from porcine heart. |
| Application: | The enzyme has been used in a primary enzyme assay to test the ability of certain compounds to reduce PDK- (pyruvate dehydrogenase kinase) catalyzed PDC (pyruvate dehydrogenase complex) inactivation. |
| Biochem/physiol Actions: | Pyruvate dehydrogenase is involved in the decarboxylation of pyruvate and reductive acetylation of lipoic acid within the pyruvate dehydrogenase complex (PDC). Pyruvate dehydrogenase kinase (PDK) inactivates pyruvate dehydrogenase while pyruvate dehydrogenase phosphatase activates pyruvate dehydrogenase. |
| Other Notes: | One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of Coenzyme A. |
| Physical form: | Supplied as a 50% glycerol solution containing ~9 mg/mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3 TRITON® X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8 |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 0.1-1.0 units/mg protein (Lowry) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |