Phosphate Buffered Saline
SIGMA/P7059 - 10× PBS for Western blots and IP
Synonym: Phosphate buffered saline; PBS
MDL Number: MFCD00131855
Product Type: Chemical
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| form | liquid |
| grade | Molecular Biology |
| pH | 7.2-7.6(1x solution) |
| Quality Level | 200  |
| sterility | 0.2 μm filtered |
| storage temp. | room temp |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: suitable |
| Application: | Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation. |
| General description: | Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications. |
| Preparation Note: | 10x PBS contains: • 30mM sodium phosphate • 1.5 M sodium chloride • 10.5 mM potassium phosphate |
| Preparation Note: | Dilute 10x PBS to a 1x working solution using molecular biology grade water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |