Protein Kinase Cν (PKCν) Active human
SIGMA/P7123 - recombinant, expressed in E. coli, ≥75% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: PKD3; Protein Kinase C nu
MDL Number: MFCD07371119
Product Type: Chemical
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| assay | ≥75% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | protein apparent mol wt ~142 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | O94806  |
| Application: | Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide (KRREILSRRPSYR) per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP. |
| Biochem/physiol Actions: | PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits activation upon BCR engagement. |
| Physical form: | Solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol |
| WGK Germany | 1 |
| Purity | ≥75% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |