Puromycin aminonucleoside
SIGMA/P7130
Synonym: 3′-Amino-3′-deoxy-N6,N6-dimethyladenosine
CAS Number: 58-60-6
Empirical Formula (Hill Notation): C12H18N6O3
Molecular Weight: 294.31
EC Number: 200-388-3
MDL Number: MFCD00063462
Linear Formula: C12H18N6O3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P7130-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P7130-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P7130-500MG
| antibiotic activity spectrum | Gram-positive bacteria |
| neoplastics | |
| parasites | |
| form | powder |
| InChI | 1S/C12H18N6O3/c1-17(2)10- |
| InChI key | RYSMHWILUNYBFW-VENHTOENSA |
| mode of action | protein synthesis | interferes |
| Quality Level | 300  |
| SMILES string | CN(C)c1ncnc2n(cnc12)[C@@H |
| solubility | H2O: soluble 50 mg/mL |
| storage temp. | 2-8°C |
| Application: | Puromycin aminonucleoside has been used: • as a selection marker for the infected cells for the transfection of lentivirus • as a component of fresh medium for transfection and infection assays • to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria |
| Biochem/physiol Actions: | Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology. |
| General description: | Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability. |
| Other Notes: | Keep container tightly closed in a dry and well-ventilated place. |
| Packaging: | 1 g in glass bottle |
| Packaging: | 100, 500 mg in glass bottle |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H373 |
| Hazard Codes | Xn |
| Risk Statements | 48/22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 51281912 |