Pyruvate Carboxylase from bovine liver
SIGMA/P7173 - buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)
Synonym: Pyruvate:CO2 ligase (ADP-forming)
CAS Number: 9014-19-1
MDL Number: MFCD00132143
Product Type: Chemical
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| concentration | ≥0.5 mg/mL |
| foreign activity | lactic dehydrogenase ≤0.5% |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | 5-25 units/mg protein (BCA) |
| storage temp. | −20°C |
| Application: | Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes. |
| Application: | The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum. |
| Biochem/physiol Actions: | Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA. |
| Other Notes: | One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C. |
| Physical form: | Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA. |
| Preparation Note: | Affinity purified |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 5-25 units/mg protein (BCA) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 6.4.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |