Pyrophosphate reagent
SIGMA/P7275 - spectrophotometric assay
Synonym: Pyrophosphate detection reagent
Product Type: Chemical
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| form | powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| usage | 1 vial sufficient for ≤4 assays |
| Analysis Note: | A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent) |
| Application: | The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts. |
| Biochem/physiol Actions: | The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH. |
| Biochem/physiol Actions: | Two moles of NADH are oxidized to NAD+ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm. The reagent can be used to detect 10-100 nmoles of pyrophosphate. |
| Other Notes: | Component Concentration Imidazole × HCl, pH 7.4 45 mM Citrate 5 mM EDTA 0.10 mM Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM β-NADH 0.8 mM F-6-P 12 mM Bovine Serum Albumin 5 mg/mL Sugar Stabilizer 5 mg/mL PPi-PFK 0.5 units/mL Aldolase 7.5 units/mL GDH 5 units/mL TPI 50 units/mL |
| Packaging: | A technical bulletin is included. |
| Preparation Note: | Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |