Polyethylene glycol/ dimethyl sulfoxide solution
SIGMA/P7306 - Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma
Synonym: PEG/DMSO Solution; Polyethylene Glycol Solution; Solution of PEG and DMSO
MDL Number: MFCD00081839
Product Type: Chemical
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| concentration | 50 % (w/v) |
| form | solution |
| grade | Hybri-Max™ |
| impurities | endotoxin, tested |
| InChI | 1S/C2H6O2/c3-1-2-4/h3-4H, |
| InChI key | LYCAIKOWRPUZTN-UHFFFAOYSA |
| mol wt | average mol wt 1,450 |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | C(CO)O |
| sterility | 0.2 μm filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization. Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO. |
| Legal Information: | Hybri-Max is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium. |
| Packaging: | Packaged in sealed ampules under nitrogen. |
| Preparation Note: | Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161902 |