PNGase F from Elizabethkingia meningoseptica
SIGMA/P7367 - BioReagent, ≥95% (SDS-PAGE)
Synonym: N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase
CAS Number: 83534-39-8
MDL Number: MFCD18839214
Product Type: Chemical
Figure 4. Cleavage site and structural requirements for PNGase F.
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
| assay | ≥95% (SDS-PAGE) |
| biological source | bacterial (Elizabethkingia meningoseptica) |
| concentration | ≥300 units/mL |
| ≥50 units/mL | |
| conjugate | (N-linked) |
| form | powder |
| mol wt | ~36 kDa |
| optimum pH | ~8.6 |
| product line | BioReagent |
| Quality Level | 200  |
| shelf life | ≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml) |
| ≥1 yr at 2‑8 °C | |
| Solution is stable for at least 3 freeze-thaw cycles | |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | PNGase F from Elizabethkingia meningoseptica has been used: • for de-N-glycosylation of Zika20virus E protein • to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells • to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA) |
| Application: | Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis |
| Application: | Used to deglycosylate protein. |
| Biochem/physiol Actions: | Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. |
| General description: | Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes. |
| Other Notes: | One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |