Carboxy-terminal FLAG-BAP™ Fusion Protein
SIGMA/P7457
MDL Number: MFCD00130921
Product Type: Chemical
| form | liquid |
| mol wt | ~49 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF). |
| Application: | Learn more product details in our FLAG® application portal. |
| Biochem/physiol Actions: | The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay. |
| General description: | Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP). |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Control protein |
| Physical form: | Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2 |
| Preparation Note: | Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |