Anti-PCAF antibody produced in rabbit
SIGMA/P7493 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-p300/CBP associated factor
MDL Number: MFCD03791929
Product Type: Chemical
Immunoblotting Recombinant human PCAF was separated on SDS-PAGE, blotted with Anti-PCAF (Cat. No. P7493) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Negative control: without antibody
Immunoblotting Nuclear extract of HeLa (lanes 1-2) and lysates of HepG2 (lanes 3-4) and Jurkat (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-PCAF (817-832) (Cat. No. P7493) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1,3,5. Antibody 2 μg/mL 2,4,6. Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)
Immunoblotting Lysate of Y79 cells was separated on SDS-PAGE, blotted with Anti-PCAF (Cat. No. P7493) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)
Immunoprecipitation Rabbit Anti-PCAF (Cat. No. P7493) was used to immunoprecipitate PCAF from an exctract of Jurkat cells. Detection antibody: Rabbit Anti-PCAF (C-terminal). Lanes 1. 5 μg/mL antibody 2. Negative control: without antibody 3. 10 μg/mL antibody 4. Negative control: without cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~88 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using RIPA lysate with ~4x107 human acute T cell leukemia Jurkat cells |
| microarray: suitable | |
| western blot (chemiluminescent): 1:500 using whole extracts of dog MDCK kidney cells | |
| UniProt accession no. | Q92831  |
| Application: | Anti-PCAF antibody is also suitable for use microarray, immunoblot, chemiluminescent western blot (1:500 using whole extracts of dog MDCK kidney cells), and immunoprecipitation (5-10 μg using RIPA lysate with approx. 4x10,000,000 human acute T cell leukemia Jurkat cells). |
| Application: | Anti-PCAF antibody produced in rabbit has been used in western blot and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-PCAF recognizes human and dog PCAF. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide. |
| Biochem/physiol Actions: | PACF (p300/CBP association factor) functions as a transcriptional coactivator in association with p300 and CBP (CREB-binding protein). PCAF share high degree of sequence similarity with human and mouse GCN5 HAT (Histone acetyltransferases). PACF mediates the acetylation of core histone. It is also known for the acetylation of non-histone proteins such as p53, MyoD, TFIIE-b, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF possesses chromatin modification activity and modulation of transcription factors for the activation of transcription. It also promotes cell growth and development as myogenesis and also contribute to receptor-mediated cell signaling or activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily. |
| Immunogen: | synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH. |
| Immunogen: | This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |