Anti-Protein Disulfide Isomerase antibody produced in rabbit
SIGMA/P7496 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Erp58; Anti-PDI
MDL Number: MFCD01094568
Product Type: Chemical
Immunofluorescence
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with Anti-Protein Disulfide Isomerase (PDI) (Cat. No. P7496) at a 1:250 dilution and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
HeLa cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with Anti-Protein Disulfide Isomerase (PDI) (Cat. No. P7496) at a 1:250 dilution and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Western Blotting Whole extract of NIH 3T3 cells were separated on SDS-PAGE and probed with Anti-Protein Disulfide Isomerase antibody produced in Rabbit (Cat. No. P7496). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 57 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, bovine, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using RIPA lysate from rat NRK cells |
| indirect immunofluorescence: 1:250 using human HeLa cells | |
| western blot: 1:2,000 using whole extract of mouse NIH3T3 cells | |
| UniProt accession no. | P07237  |
| Application: | Anti-Protein Disulfide Isomerase antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence. |
| Biochem/physiol Actions: | Prolyl 4-hydroxylase subunit β (P4HB) or protein disulfide isomerase acts as a molecular chaperone in the endoplasmic reticulum of cells and also as an oxidoreductase. It associates with steroid hormones and modulates their actions, concentrations and storage. P4HB accelerates the formation of disulphide bonds in proteins and hence aids in their folding. |
| Biochem/physiol Actions: | Protein Disulfide Isomerase (PDI) catalyzes the formation and rearrangements of both intrachain and interchain disulfide bonds in secreted proteins. It plays an important role in various cellular processes including cell adhesion. PDI has calcium-dependent transglutaminase activity and is involved in the catalysis of isopeptide bond formation. PDI participates in the hydroxylation of proline in procollagen during collagen synthesis and in the transfer of neutral lipid onto nascent lipoprotein particles. Estrogen binding by PDI has also been reported. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Prolyl 4-hydroxylase subunit β (P4HB) or protein disulfide isomerase is a redox-regulated thiol-containing protein. The gene encoding this protein is localized on human chromosome 17q25.3. |
| General description: | Protein Disulfide Isomerase (PDI, Erp58) is an abundant multifunctional, soluble enzyme (E.C. 5.3.4.1) that resides in the lumen of the endoplasmic reticulum of eukaryotic cells. It consists of four tandem domains, two of which contain a catalytic site for disulfide bond formation. A mitochondrial isoform of PDI (approx. 54 kDa) has been recently described. PDI has an N-terminal endoplasmic reticulum (ER) signal and a C-terminal ER- retention KDEL signal sequences. PDI was found on the cell surface of several cell types including endothelial cells, platelets and hepatocytes. |
| Immunogen: | protein disulfide isomerase purified from bovine liver. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |