Amino-terminal FLAG-BAP™ Fusion Protein
SIGMA/P7582
Synonym: Amino-terminal Fusion Protein; BAP Fusion Protein; FLAG-BAP Fusion Protein
MDL Number: MFCD00130921
Product Type: Chemical
| form | liquid |
| mol wt | ~49 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome. |
| Application: | Learn more product details in our FLAG® application portal. |
| General description: | Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins. |
| General description: | The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS. |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Control protein |
| Physical form: | Protein is supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2 in 50% glycerol, pH 8.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |