Monoclonal Anti-Protein Phosphatase 1α antibody produced in mouse
SIGMA/P7607 - clone PPI-377, ascites fluid
Synonym: Anti-PP1α
MDL Number: MFCD02097274
Product Type: Chemical
Western Blotting Whole extract of NIH-3T3 Mouse fibroblasts cells were separated on SDS-PAGE and probed with Monoclonal Anti-Protein Phosphatase 1-ALPHA antibody produced in Mouse, Clone: PPI-377 (Cat. No. P7607). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. Negative Control 2. 1:500 antibody 3. 1:1,000 antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PPI-377, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| mol wt | antigen 37.5 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, rat, bovine, human, mouse, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1:500 using mouse fibroblasts cell extract | |
| UniProt accession no. | P62136  |
| Application: | Anti-Protein Phosphatase 1α is suitable for immunoblotting at a working dilution of 1:500 using mouse fibroblasts cell extract. A dilution of 1:200 has been used for immunoblotting in myocardial protein extracts of Göttinger minipigs. For immunoprecipitation, 1 μg antibody/20 μg of pig protein extracts was suitable. The antibody is also suitable for immunocytochemistry and protein microarray. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | The antibody reacts with protein phosphatase 1α and recognizes an epitope within its catalytic subunit. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. PP1 catalyzes a wide range of protein dephosphorylation reactions in a tightly regulated manner and is expressed abundantly in the brain. PP1 has broad functions covering glycogen metabolism, protein synthesis, cell cycle and growth and muscle contractility. PP1 forms exclusive complexes with >50 regulatory subunits that allows for restricted subcellular location and thereby distinct cellular functions. There are 3 isoforms of PP1 (α, β and γ1) with 90% homology. PP1α is expressed in brain, specifically in cerebellum, prefrontal cortex and synapses Monoclonal Anti-Protein Phosphatase 1α specifically recognizes an epitope within the catalytic subunit of PP1α isoform (37.5 kDa). |
| Immunogen: | recombinant rabbit protein phosphatase 1α (PP1α) catalytic subunit |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |