PKD2 Active human
SIGMA/P7623 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution
Synonym: Protein Kinase D2
MDL Number: MFCD07371065
Product Type: Chemical
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| assay | ≥70% (SDS-PAGE) |
| form | aqueous glycerol solution |
| mol wt | protein apparent mol wt ~140 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | 100 units/mg protein (Bradford) |
| storage temp. | −70°C |
| UniProt accession no. | Q9BZL6  |
| Application: | Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP. |
| Biochem/physiol Actions: | PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation. |
| Other Notes: | One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP. |
| Physical form: | Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol. |
| WGK Germany | 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 100 units/mg protein (Bradford) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |