Anti-Profilin 1 (C-terminal) antibody produced in rabbit
SIGMA/P7624 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-PFN1
MDL Number: MFCD09265368
Product Type: Chemical
Immunoprecipitation
Lane 1. Positve control — Whole extract of Mouse 3T3 cells was separated on SDS-PAGE and blotted with Anti-Profilin 1 (C-terminal) (Cat. No. P7624)
Lane 2. Immunoprecipitation — 10 μg of Anti-Profilin 1 (C-terminal) (Cat. No. P7624) was used to immunoprecipitate mouse profilin 1 from a 3T3 cell lysate. Detection antibody: Anti-Profilin 1 (N-terminal) (Cat. No. P7749)
Lane 3. Negative control — non-relevant antibody was used for IP. Detection antibody: Anti-Profilin 1 (N-terminal) (Cat. No. P7749)
Lane 1. Positve control — Whole extract of Mouse 3T3 cells was separated on SDS-PAGE and blotted with Anti-Profilin 1 (C-terminal) (Cat. No. P7624)
Lane 2. Immunoprecipitation — 10 μg of Anti-Profilin 1 (C-terminal) (Cat. No. P7624) was used to immunoprecipitate mouse profilin 1 from a 3T3 cell lysate. Detection antibody: Anti-Profilin 1 (N-terminal) (Cat. No. P7749)
Lane 3. Negative control — non-relevant antibody was used for IP. Detection antibody: Anti-Profilin 1 (N-terminal) (Cat. No. P7749)
Immunofluorescence NRK Rat kidney cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Anti-Profilin 1 (C-terminal) antibody produced in Rabbit (Cat. No. P7624). The antibody was developed using 1:200 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~15 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates |
| indirect immunofluorescence: 10-20 μg/mL using rat NRK cells | |
| western blot (chemiluminescent): 1-2 μg/mL using whole extract of human HeLa cells | |
| UniProt accession no. | P15735  |
| Application: | Anti-Profilin 1 (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunostaining • immunoprecipitation • immunofluorescence • profilin enzyme-linked immunosorbent assay (ELISA) analysis |
| Biochem/physiol Actions: | Profilin 1 is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype. Profilin 1 is a potent regulator of actin filament dynamics. Although profilin 1 prevents spontaneous actin polymerization by complexing with unpolymerized actin in vivo, actin-profilin complexes can be added to free barbed ends, thereby stimulating actin polymerization. Profilin 1 was suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over-expressed in breast cancer cells. Deletion of profilin 1 is associated with Miller-Dieker syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Profilin 1 is a ubiquitous actin monomer-binding protein. Profilin 1 is the most ubiquitous and abundant and is highly expressed throughout development and adulthood in most tissues including brain. |
| General description: | Profilin 1 is a ubiquitous actin monomer-binding proteininvolved in actin polymerization in response toextracellular signals. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 128-140 of human profilin 1, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |