Phenylmethanesulfonyl fluoride
SIGMA/P7626 - ≥98.5% (GC)
Synonym: α-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride; PMSF; Phenylmethylsulfonyl fluoride
CAS Number: 329-98-6
Empirical Formula (Hill Notation): C7H7FO2S
Molecular Weight: 174.19
EC Number: 206-350-2
MDL Number: MFCD00007424
Linear Formula: C7H7FO2S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P7626-1G
| assay | ≥98.5% (GC) |
| biological source | synthetic |
| form | powder |
| InChI | 1S/C7H7FO2S/c8-11(9,10)6- |
| InChI key | YBYRMVIVWMBXKQ-UHFFFAOYSA |
| mp | 91-94 °C |
| Quality Level | 300  |
| SMILES string | FS(=O)(=O)Cc1ccccc1 |
| solubility | dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.) |
| H2O: unstable |
| Application: | Phenylmethanesulfonyl fluoride has been used in following applications: • cell fractionation. • used as a supplement in nuclear protein extraction. • inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE). • used for the collection of blood prior to centrifugation to quantify plasma ANP levels. |
| Application: | PMSF is often used in lysis buffer to assist in preserving proteins of interest during protein isolation and sample preparation, by inhibiting proteases that would otherwise degrade proteins after cell or tissue lysis. PMSF is unstable in aqueous media. Thus stock solutions of PMSF are generally prepared in anhydrous organic solvents (e.g. 100% ethanol, or anhydrous isopropanol) prior to use in aqueous media. Noted general features and benefits of PMSF include the following: • Inhibits serine proteases such as trypsin and chymotrypsin • Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase • Not as effective or as toxic as DFP • Effective concentration 0.1-1 mM • Half-life = 1 hr. at pH 7.5 |
| Biochem/physiol Actions: | Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin. |
| General description: | Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor, effective against such enzymes as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor via sulfonation of the hydroxyl residues of serine residues at the reactive sites of serine proteases. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 1, 5, 25, 100 g in glass bottle |
| Packaging: | 250 mg in glass bottle |
| Symbol |   GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H301 - H314 |
| Precautionary statements | P260 - P270 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 25-34 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 2928 8(6.1) / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98.5% (GC) |
| mp | 91-94 °C |
| UNSPSC | 12352202 |