Phospholipase C from Clostridium perfringens (C. welchii)
SIGMA/P7633 - Type I, lyophilized powder, 10-50 units/mg protein
Synonym: Lecithinase C; Lipophosphodiesterase I; PC-PLC; Phosphatidylcholine cholinephosphohydrolase
CAS Number: 9001-86-9
EC Number: 232-638-2
MDL Number: MFCD00131895
Product Type: Chemical
Degumming of feedstock oils by hydrolyisis of phospholipids can be catalyzed by phospholipase C. Treatment of
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
phosphatidylethanol, phosphatidylcholine and phosphatidylinositol with phospholipase C enables the hydrolysis
and phase separation of the hydrophilic phosphate-containing contaminants.
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial (Clostridium perfringens) |
| composition | protein, 20-40% |
| form | lyophilized powder |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| specific activity | 10-50 units/mg protein |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| type | Type I |
| Application: | Phospholipase C (PLC) has been used to study adrenoceptor-mediated transmembrane signaling. It is also used to degrade inositol-containing phospholipids. Furthermore, it has been used to study 9E3 gene activation. |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. |
| Biochem/physiol Actions: | PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 10-50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |