3-Phosphoglyceric Phosphokinase from baker’s yeast (S. cerevisiae)
SIGMA/P7634 - ammonium sulfate suspension, ≥500 units/mg protein
Synonym: PGK; Phosphoglycerate kinase; 3-Phosphoglycerate kinase; ATP:3-Phospho-D-glycerate 1-phosphotransferase
CAS Number: 9001-83-6
EC Number: 232-636-1
MDL Number: MFCD00130301
Product Type: Chemical
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| biological source | bakers yeast |
| concentration | 1.0-10.0 mg/mL |
| foreign activity | Glyceraldehyde-3-phosphat |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥500 units/mg protein |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by TCA Biuret. |
| Application: | 3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae) has been used: • to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphat • in the coupled assay to measure the backward activity of purified rabbit skeletal muscle nicotinamide adenine dinucleotide (NAD+)-dependent glyceraldehyde3-phosphate dehydrogenase (GAPDH) • in the assay of glyceraldehyde-3-phosphat |
| Biochem/physiol Actions: | 3-Phosphoglyceric phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate phosphokinase activity is essential for glycolysis and gluconeogenesis. Phosphoglyceratekinase (PGK) plays a vital role in the glycolytic pathway by catalyzing one of the two ATP-producing reactions. It converts 1,3-bisphosphoglycerate (1,3BPGA) to 3-phosphoglycerate (3PGA). Additionally, it takes part in the process of gluconeogenesis by catalyzing the opposite reaction to create 1,3BPGA and adenosine diphosphate (ADP). Due to its participation in numerous processes other than energy metabolisms, such as pathogenesis, interaction with nucleic acids, tumorigenesis progression, cell death, and viral replication, PGK is also known as a moonlighting protein. |
| General description: | Research area: Cell Signaling Phosphoglycerate kinase (PGK), a glycolytic enzyme, is isolated from a broad variety of organisms. This typical hinge-bending monomeric enzyme is well-conserved among the three domains of life. The enzyme is made up of a single folded polypeptide chain that divides into two nearly identical domains, each linked by two -helices (-helices 7 and 14) and separated by a deep cleft. This arrangement gives the enzyme its distinctive bilobed structure. |
| Other Notes: | One unit will convert 1.0 μmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C. |
| Physical form: | Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0 |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.2.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |