Pyruvate Kinase from rabbit muscle
SIGMA/P7768 - Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein
Synonym: ATP:pyruvate 2-
CAS Number: 9001-59-6
MDL Number: MFCD00081946
Product Type: Chemical
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| concentration | 2.0-20.0 mg/mL |
| foreign activity | lactic dehydrogenase and creatine phosphokinase ≤0.01% |
| phosphoglucomutase and myokinase ≤0.05% | |
| form | buffered aqueous glycerol solution |
| mol wt | 237 kDa |
| Quality Level | 300  |
| specific activity | 350-600 units/mg protein |
| storage temp. | 2-8°C |
| type | Type VII |
| Analysis Note: | Protein determined by biuret. |
| Application: | Pyruvate Kinase from rabbit muscle has been used as a component in refolding buffer for malate dehydrogenase (MDH) assay. It has also been used as a supplement in assay buffer for phosphoglycerate mutases (PGM) enzyme assays. |
| Application: | Pyruvate kinase from Sigma has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. |
| Biochem/physiol Actions: | Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa. Isoelectric Point: 7.6 Optimal pH: ∼7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. |
| Biochem/physiol Actions: | Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP. Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa. Isoelectric Point: 7.6 Optimal pH: ∼7.5 |
| Biochem/physiol Actions: | Pyruvate kinase is an enzyme involved in glycolysis and gluconeogenesis. Product P7768 is from rabbit muscle and is provided in a buffered aqueous glycerol solution and has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency |
| General description: | Research Area: Cell Signaling Pyruvate kinase plays a role in regulating cell metabolism. There are four pyruvate kinase isoforms in mammals (PKM1, PKM2, PKR, PKL). Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits, arranged in a dimer-of-dimers configuration. Each monomer contains one active site and consists of three main domains- designated A, B, and C-along with a small N-terminal domain. The M2 isoform of pyruvate kinase (PKM2) supports anabolic metabolism and is expressed in cancer and normal tissue. |
| Other Notes: | One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C. |
| Physical form: | Solution in 50% glycerol containing 0.01 M phosphate, pH 7.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 350-600 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.1.40 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |