Anti-Presenilin-1 (S182) antibody produced in rabbit
SIGMA/P7854 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-ACNINV3; Anti-AD3; Anti-FAD; Anti-PS-1; Anti-PS1; Anti-S182
MDL Number: MFCD02097229
Product Type: Chemical
Immunohistochemistry Staining of methacarn-fixed, paraffin-embedded, formic acid-treated Alzheimer′s Disease (AD) brain sections, with 1:100 Rabbit Anti-Presenilin-1 (S182) (Cat. No. P7854) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell line lysate was separated on SDS-PAGE and probed with 1:10,000 Rabbit Anti-Presenilin-1 (S182) (Cat. No. P7854). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. PC-12
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, Xenopus, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using methacarn-fixed, formic acid-treated sections of Alzheimer′s Disease (AD) brain |
| microarray: suitable | |
| western blot: 1:10,000 using whole cell extract of PC12 rat adrenal pheochromocytoma cell line | |
| UniProt accession no. | P49768  |
| Application: | Anti-Presenilin-1 (S182) antibody produced in rabbit has been used in: • immunostaining • immunofluorescence detection • immunohistochemistry • immunoblotting |
| Biochem/physiol Actions: | Specific for presenilin 1 (not found in presenilin 2) and is identical in mouse, rat, and Xenopus. By immunoblotting, the antibody reacts with presenilin 1 (PS1, S182) and with the PS1 cleavage product. |
| Biochem/physiol Actions: | The biological functions of the presenilin′s are yet unknown, but they are thought to be involved in protein trafficking. Mutations in the presenilin 1 (PS1) gene (>30 known pathogenic mutations) have been found in about half of the early-onset familial Alzheimer′s disease (FAD), while only two pathogenic mutations have been found in PS2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Presenilin 1& 2 (PS1 and PS2) are highly homologous proteins (approx. 43-52 kD) with eight transmembrane domains. In the brain, PS1 and PS2 are expressed primarily in neurons and localized mainly in cell bodies, axons and dendrites. In non-neuronal cells, PS1 and PS2 are localized to the nuclear membrane, endoplasmic reticulum and Golgi. Presenilin 1 is located on chromosome 14. |
| Immunogen: | synthetic peptide corresponding to the C-terminal of human presenilin-1 (amino acids 450-467 with N-terminally added lysine) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |