Anti-Peroxidase antibody produced in rabbit
SIGMA/P7899 - fractionated antiserum, lyophilized powder
Synonym: Rabbit Anti HRP
MDL Number: MFCD00162388
Product Type: Chemical
ELISA Anti-Peroxidase antibody produced in Rabbit (Cat. No. P7899), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919), was serially diluted to detect Horsedise Peroxidase by indirect ELISA.
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | horseradish |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:100,000-1:200,000 |
| Analysis Note: | Specificity is determined by immunoelectrophoresis using a crude peroxidase extract. |
| Application: | Anti-Peroxidase antibody produced in rabbit has been used in: • immunoblot assays • enzyme-linked-immunosor • immunoprecipitation |
| Biochem/physiol Actions: | Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase. |
| General description: | Peroxidase is a hemoprotein. |
| General description: | The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets. |
| Immunogen: | Purified peroxidase from horseradish |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.2 |
| Preparation Note: | Reconstitute with deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |