Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit
SIGMA/P7937 - 5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder
Synonym: Protein phosphatase
MDL Number: MFCD01635658
Product Type: Chemical
Unit Definition One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30°C.
| assay | ≥80% (GE) |
| biological source | rabbit |
| form | lyophilized powder |
| mol wt | 37.5 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | 5,000-15,000 units/mg protein |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P62139  |
| Application: | Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay. |
| Application: | Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts. |
| Biochem/physiol Actions: | Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn2+ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2). |
| General description: | Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M). |
| Other Notes: | One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C. |
| Packaging: | 25 μg in glass bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl2, Tween 20, and trehalose as stabilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% (GE) |
| activity | specific activity: 5,000-15,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.3.16 ( BRENDA | IUBMB ) |
| UNSPSC | 12352202 |