Monoclonal Anti-PSTAIR antibody produced in mouse
SIGMA/P7962 - clone PSTAIR, ascites fluid
Synonym: Anti-CDC2
MDL Number: MFCD00677382
Product Type: Chemical
Western Blotting JOURNAL CITATION: Fission yeast CSL proteins function as transcription factors. By: Oravcová, M., Teska, M., et al. in PLoS One, 2013. PubMed ID: 23555033 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0059435), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-PSTAIR Clone:PSTAIR (Cat. No. P7962). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SK-N-SH 4. SH-SY5Y 5. U87 6. COS7 7. PC12 8. Neuro-2a 9. Arabidopsis
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PSTAIR, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 33-34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1:4,000 using a COS-7 cell extract |
| Application: | Monoclonal Anti-PSTAIR antibody produced in mouse has been used in: • western blot analysis • enzyme-linked immunosorbent assay (ELISA) • immunoprecipitation |
| Application: | Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation. |
| Biochem/physiol Actions: | Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms. |
| General description: | Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21. |
| Immunogen: | synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |