Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA
SIGMA/P7998 - ready to use solution
Synonym: Alkaline phosphatase substrate solution (pNPP)
Product Type: Chemical
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.
| form | liquid |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications. |
| Disclaimer: | Stable at least one year at 2-8 °C. |
| General description: | The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications. |
| Physical form: | Ready to use |
| Preparation Note: | Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase. |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H318 - H373 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 41-48/22 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |