Proteinase, bacterial
SIGMA/P8038 - Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonym: Proteinase from Bacillus licheniformis; Alkaline Protease; Protease from Bacillus licheniformis; Proteinase from Bacillus licheniformis; Subtilo peptidase A; Alkaline Protease; Protease from Bacillus licheniformis; Subtilo peptidase A
CAS Number: 9014-01-1
EC Number: 232-752-2
MDL Number: MFCD00132092
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8038-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8038-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8038-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8038-50MG
| form | lyophilized powder |
| mol wt | 27 kDa |
| purified by | crystallization |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | ≤0.05 Kunitz units/mg solid RNase |
| ≤5.0 Kunitz units/mg solid DNase | |
| 7.0-14.0 units/mg solid | |
| storage temp. | −20°C |
| type | Type XXIV |
| Analysis Note: | Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg. |
| Application: | The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA. |
| Application: | This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1. |
| Biochem/physiol Actions: | Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions. |
| Biochem/physiol Actions: | Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat |
| General description: | Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa. |
| Other Notes: | One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 1 g in glass bottle |
| Packaging: | 50, 100, 250 mg in glass bottle |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H334 - H335 - H410 |
| Precautionary statements | P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-41-42-50 |
| Safety Statements | 22-24-26-36/37/39-61 |
| RIDADR | UN 3082 9 / PGIII |
| WGK Germany | WGK 1 |
| activity | specific activity: ≤0.05 Kunitz units/mg solid RNase; specific activity: ≤5.0 Kunitz units/mg solid DNase; specific activity: 7.0-14.0 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.62 ( BRENDA  | IUBMB ); 3.4.21.62 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |