Proteinase K from Tritirachium album
SIGMA/P8044 - ≥3.0 unit/mg solid, lyophilized powder
Synonym: Endopeptidase K
CAS Number: 39450-01-6
EC Number: 254-457-8
MDL Number: MFCD00132129
Product Type: Chemical
Proteinase K illustration.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8044-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8044-250MG
| application(s) | diagnostic assay manufacturing |
| biological source | maize |
| microbial (T.album T. ALBUM) |
|
| plant seeds (barley) | |
| soybean | |
| yeast | |
| composition | Protein, ≥15% biuret |
| form | lyophilized powder |
| mol wt | 28.93 kDa |
| Quality Level | 200  |
| specific activity | ≥3.0 unit/mg solid |
| storage temp. | −20°C |
| technique(s) | DNA extraction: suitable |
| Application: | Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. It is also useful for the isolation of hepatic, yeast, and mung bean mitochondria and is used to determine enzyme localization on membranes. Furthermore, it is used for the treatment of paraffin embedded tissue sections to expose antigen binding sites and for digestion of proteins from brain tissue samples. Product P8044 is provided as a lyophilized powder. |
| Application: | The enzyme from Sigma has been used to degrade complex I (NADH:ubiquinone oxidoreductase) prior to extraction of ubiquinone from Yarrowia lipolytica. It has been used to examine the effect of Proteinase k on Pythium ultimum. It has also been used to maximize the variety of peptide bonds hydrolyzed in the sediment slurry without autolytic production of amino acids from the enzyme itself. |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. |
| Application: | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
| Biochem/physiol Actions: | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
| Biochem/physiol Actions: | Proteinase K is highly active towards native proteins. It has a broad specificity and degrades many proteins even in the native state. It mainly cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked α-amino groups. The optimum pH is between 7.5-9.0 and the isoelectric point is 8.9. Ca2+ (1-5 mM) is required for activation. Proteinase K is inhibited by diisopropyl fluorophosphate (DFIP), and phenylmethanesulfonyl fluoride (PMSF). |
| Other Notes: | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 250 mg in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 |
| Precautionary statements | P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥3.0 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.64 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |