Protein G-Biotin from Streptococcus sp.
SIGMA/P8045 - recombinant, expressed in unspecified host, lyophilized powder
Synonym: Biotinylated Protein G; Protein G Biotin; Protein G-Biotin
MDL Number: MFCD00132111
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| composition | Protein, ~90% biuret |
| conjugate | biotin conjugate |
| extent of labeling | 2-4 mol d-biotin per mol protein |
| form | lyophilized powder |
| Quality Level | 200  |
| recombinant | expressed in unspecified host |
| storage temp. | 2-8°C |
| technique(s) | direct ELISA: 1:70,000 using human IgG |
| Application: | Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads. |
| Application: | Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities. |
| Biochem/physiol Actions: | Binds IgG only from most mammalian species except cat; binds chicken IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein. |
| General description: | Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos. |
| Other Notes: | Truncated - lacks albumin, Fab and membrane binding sites. |
| Packaging: | Package size based on protein content |
| Preparation Note: | Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |