Phytagel™
SIGMA/P8169 - BioReagent, suitable for plant cell culture, powder
Synonym: Gellan Gum; Agar substitute gelling agent
CAS Number: 71010-52-1
EC Number: 275-117-5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8169-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8169-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8169-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8169-1KG
| application(s) | agriculture |
| form | powder |
| product line | BioReagent |
| Quality Level | 200  |
| technique(s) | cell culture | plant: suitable |
| Application: | Phytagel™ has been used: • in the solidification of culture medium for root assays in Arabidopsis thaliana • as a component of Solid Paul′s medium for Agrobacterium culture • as a component of root induction medium in Ilex dumosa tissue culture |
| Biochem/physiol Actions: | Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets. |
| General description: | Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel. |
| Legal Information: | Phytagel is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination. |
| Packaging: | 1, 5 kg in poly drum |
| Packaging: | 100, 250, 500 g in poly bottle |
| Preparation Note: | To prevent clumping, add slowly to rapidly stirring medium prior to heating. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 10171502 |