PIPES

SIGMA/P8203 - BioXtra, ≥99% (titration)

Synonym: 1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperazine-N,N′-bis(2-ethanesulfonic acid)

CAS Number: 5625-37-6
Empirical Formula (Hill Notation): C₈H₁₈N₂O₆S₂
Molecular Weight: 302.37
EC Number: 227-057-6
MDL Number: MFCD00006159
Linear Formula: C₈H₁₈N₂O₆S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-P8203-50G	50 g	$265.00 1/EA	Add To Favorites
45-P8203-250G	250 g	$1010.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: P8203-50G

Properties
Descriptions
Safety Info.
Basic Data

absorption	≤0.1 at 260 in 1 M NaOH at 0.5 M
	≤0.1 at 280 in 1 M NaOH at 0.5 M
anion traces	chloride (Cl^-): ≤0.2%
application(s)	diagnostic assay manufacturing
assay	≥99% (titration)
cation traces	Al: ≤0.005%
	Ba: ≤0.0005%
	Bi: ≤0.0005%
	Ca: ≤0.005%
	Cd: ≤0.0005%
	Co: ≤0.0005%
	Cr: ≤0.0005%
	Cu: ≤0.0005%
	Fe: ≤0.0005%
	K: ≤0.005%
	Li: ≤0.0005%
	Mg: ≤0.0005%
	Mn: ≤0.0005%
	Mo: ≤0.0005%
	Na: ≤0.1%
	Ni: ≤0.0005%
	Pb: ≤0.0005%
	Sr: ≤0.0005%
	Zn: ≤0.0005%
form	powder
ign. residue (900°C)	≤0.5% (as SO₄)
impurities	Insoluble matter, passes filter test
InChI	1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
InChI key	IHPYMWDTONKSCO-UHFFFAOYSA-N
loss	≤0.5% loss on drying, 110°C
mp	>300 °C (lit.)
pKa (25 °C)	6.8
product line	BioXtra
Quality Level	400
SMILES string	OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
solubility	1 M NaOH: 0.5 M at 20 °C, clear, colorless
useful pH range	6.1-7.5

Analysis Note:	Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.
Application:	Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.^, PIPES has been utilized in protein crystallization.^, The use of PIPES in the reconstitution of dissociated tubulin α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8. A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.
General description:	PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.
Other Notes:	Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851 ) as well as several different salts for convenience in buffer preparation.
Packaging:	50, 250 g in poly bottle
Preparation Note:	Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (titration)
mp	>300 °C (lit.)
UNSPSC	12161700

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