Protease Inhibitor Cocktail
SIGMA/P8215 - for use with fungal and yeast extracts, DMSO solution
Synonym: Protease Inhibitor Mix; Protease inhibitor
EC Number: 200-664-3
MDL Number: MFCD00677817
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8215-1ML
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8215-5ML
| form | DMSO solution |
| Quality Level | 300  |
| solubility | water: soluble |
| storage temp. | −20°C |
| Application: | Protease Inhibitor Cocktail has been used as a component in: • FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip) • potassium phosphate buffer (pbs) to digest chitin • extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis |
| Biochem/physiol Actions: | Inhibits serine, cysteine, aspartic, and metalloproteases. |
| Features and Benefits: | • Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts. • Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain. • Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage. • Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays. • Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results. |
| General description: | The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast. |
| Other Notes: | AEBSF, 100 mM E-64, 1.4 mM Pepstatin A, 2.2 mM 1,10-Phenanthroline, 500 mM |
| Other Notes: | Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesu |
| Other Notes: | This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. |
| Packaging: | 1, 5 mL in glass bottle |
| Physical form: | Solution in DMSO |
| Preparation Note: | One mL is recommended for the inhibition of proteases extracted from 20 g of yeast. |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H302 - H410 |
| Precautionary statements | P264 - P270 - P273 - P301 + P312 - P391 - P501 |
| Risk Statements | 36/38 |
| Safety Statements | 24-26 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |