Anti-P2X7 Purinergic Receptor antibody produced in rabbit
SIGMA/P8232 - affinity isolated antibody, lyophilized powder
Synonym: Anti-P2X₇ Antibody; P2X₇ Receptor Detection; Rabbit Anti-P2X₇
MDL Number: MFCD02097118
Product Type: Chemical
Immunohistochemistry Anti-P2X7 Receptor: Cat. No. P8232: Immunohistochemical staining of Rat paraffin-embedded endocrine and exocrine pancreas sections using Anti-P2X7 Receptor antibody, (1:50), followed by Goat Anti-Rabbit-AlexaFluor-555-secondary antibody (1:500), (red). Staining is highly specific for endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain (blue).
Immunoblotting Anti-P2X7 Receptor: Cat. No. P8232: Western blot analysis of Rat hippocampus lysates. Lanes 1. Anti-P2X7 Receptor antibody, (1:200). 2. Anti-P2X7 Receptor antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:200-1:1,000 | |
| UniProt accession no. | Q99572  |
| Application: | Anti-P2X7 Purinergic Receptor antibody produced in rabbit has been used in • immunoprecipitation • immunofluorescence and confocal microscopy • western blotting |
| Biochem/physiol Actions: | Purinergic receptor P2X7 has a role in immunological processes. It has a role during oxidative stress under pathological conditions. The channel is permeable to Na+, Ca2+ and K+. Prolonged activation of the channel under some pathological conditions leads to the passage of large molecules and ultimately cell death. P2X7 may be involved in the release of interleukin-1β (IL-1β) and indirectly initiate an inflammatory cascade. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Purinergic receptor P2X7 is an ATP-gated cation channel. It is expressed in retinal pigmented epithelium and neural retina. The gene encoding this protein is localized on human chromosome 12. |
| Immunogen: | Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 Receptor (Accession Q64663). Intracellular, C-terminus. |
| Other Notes: | In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide. |
| Preparation Note: | Prior to reconstitution, store at –20°C. After reconstitution, the stock antibody solution may be stored at 2–8°C for up to two weeks. For extended storage, freeze at -20°C, or below, in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. Centrifuge all antibody preparations before use (10,000 x g 5 min). Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |