Protocatechuate 3,4-Dioxygenase from Pseudomonas sp.
SIGMA/P8279 - lyophilized powder, ≥3 units/mg solid
CAS Number: 9029-47-4
MDL Number: MFCD00132132
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. Stability (liquid form at 25 °C); buffer composition:50 mM Tris-HCl buffer, pH 7.8; containing 0.1% NaN3
Figure 3. pH Activity; 37 °C, in 50 mM buffer solution: pH 6.0–8.0, Tris-acetate; pH 7.5–10.5, glycine-NaOH
Figure 4. Temperature activity; in 50 mM K-phosphate buffer, pH 7.5
Figure 5. pH Stability; 25 °C, 72-hr treatment with 50 mM buffer solution: pH 3.3–5.5, acetate; pH 5.5–8.0, K-phosphate; pH 6.5–9.0, Tris-acetate
Figure 6. Thermal stability; 1-hr treatment with 50 mM K-phosphate buffer, pH 6.0
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8279-25UN
| biological source | bacterial (Pseudomonas spp.) |
| form | lyophilized powder |
| mol wt | ~700 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥3 units/mg solid |
| storage temp. | −20°C |
| Analysis Note: | Protein determined by biuret. |
| Application: | Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae. |
| Application: | The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay. |
| Biochem/physiol Actions: | Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate. |
| General description: | Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe3+. |
| General description: | Structure : Protein with nonheme iron Inhibitors : Ag+, Hg++, PCMB Optimum pH : 9.0 Optimum temperature : 60−65°C pH Stability : pH 7.0−9.0 (25°C, 72hr) Thermal stability : below 50°C (pH 6.0, 1hr) |
| Other Notes: | One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C. |
| Packaging: | 25 units in glass bottle |
| Physical form: | Supplied as lyophilized powder. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥3 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.13.11.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |