Phospholipase D from cabbage
SIGMA/P8398 - Type IV, lyophilized powder, ≥100 units/mg solid
Synonym: Lecithinase D; Phosphatidylcholine phosphatidohydrolase; Phospholipase D from white cabbage
CAS Number: 9001-87-0
EC Number: 232-639-8
MDL Number: MFCD00131897
Product Type: Chemical
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| composition | Protein, ~25% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥100 units/mg solid |
| storage temp. | −20°C |
| type | Type IV |
| Analysis Note: | Protein determined using biuret. |
| Application: | Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies. |
| Application: | The enzyme has been used for the preparation of Bodipy-phosphatidylcholin |
| Biochem/physiol Actions: | Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
| Biochem/physiol Actions: | Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions. |
| Biochem/physiol Actions: | The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
| General description: | Phospholipase D (PLD) from cabbage comprises the C2 domain, PLD superfamily specific conserved regions (I–IV) with HxKx4Dx6GSxN (HKD) motif. Structurally, it is a barrel-shaped monomer bound to calcium ions. |
| Other Notes: | One unit will liberate 1.0 μmol of choline from |
| Packaging: | 2500 units in poly bottle |
| Packaging: | 500 units in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| activity | specific activity: ≥100 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.4.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |