Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
SIGMA/P8804 - buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
Synonym: 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming; PI-PLC
CAS Number: 37288-19-0
EC Number: 232-638-2
MDL Number: MFCD00131896
Product Type: Chemical
Figure 10. General structure of GPI anchored proteins. The lipid tails of the phosphatidylinositol moiety are shown inserted through the outer cell membrane and held by the lipid bilayer.
| biological source | Bacillus sp. (Bacillus cereus) |
| foreign activity | Phopholipase C (lecithinase) ≤1 units/mg protein |
| Sphingomyelinase ≤40 units/mg protein | |
| form | buffered aqueous glycerol solution |
| mol wt | 28 kDa |
| Quality Level | 200  |
| specific activity | ≥1,000 units/mg protein (Lowry) |
| storage temp. | 2-8°C |
| Analysis Note: | Acetylcholinesterase is measured according to Ellman, et al. |
| Application: | Phospholipase C, Phosphatidylinositol-spec • in the hydrolysis of substrates p-nitrophenylphosphorylch • to cleave glycosylphosphatidylinosi • to cleave immunolabeled HeLa cells |
| Biochem/physiol Actions: | Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinosi |
| Biochem/physiol Actions: | Used for the release of GPI anchored proteins from the membrane. |
| General description: | Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein. |
| Other Notes: | One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation). |
| Packaging: | 5, 25 units in poly bottle |
| Physical form: | Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 units/mg protein (Lowry) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.6.1.13 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |