Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse
SIGMA/P8825 - clone PC 10, ascites fluid
Synonym: Monoclonal Anti-Proliferating Cell Nuclear Antigen; Anti-PCNA
Product Type: Chemical
Western Blotting JOURNAL CITATION: STAMP2 increases oxidative stress and is critical for prostate cancer. By: Jin, Y., Wang, L., et al. in EMBO Mol Med, 2015. PubMed ID: 25680860 Image collected and cropped by CiteAb from the following publication, (http://embomolmed.embopress.org/cgi/doi/10.15252/emmm.201404181), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Human Tonsil stained with Anti-CD3, T Cell (Cat. No. C7930) at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen (Cat. No. P8825) at a 1:500 dilution (green). Counterstained with DAPI. From Pamela Wirth, Plastic Surgery Department, Vanderbilt University, Nashville, TN.
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:3,000 Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in Mouse, Clone: PC 10 (Cat. No. P8825), followed by 10 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 10 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of HS-68 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in Mouse, Clone: PC 10 (Cat. No. P8825). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1293). Lanes 1. 1:3,000 antibody 2. 1:6,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PC 10, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey, insect, yeast |
| rat, mouse | |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:3,000 using HS-68 human foreskin- cell extract | |
| UniProt accession no. | P04961  |
| P12004 |
| Application: | Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used for immunohistochemistry (IHC) Western blotting (WB). |
| Application: | Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used in: • enzyme linked immuno sorbent assay (ELISA) • immunoblotting • immunohistology • immunoprecipitation • flow cytometry |
| Biochem/physiol Actions: | PCNA (proliferating cell nuclear antigen) is loaded around the template-primer 3′ ends, which is recognized by the conserved chaperone-like complex RFC (replication factor C), and this mechanism is ATP-dependent. Encircling of the DNA by the PCNA ring ensures firm anchoring of the polymerases to the DNA, and hence, functions as a co-factor for DNA polymerases. Post-translation modifications of this protein at the K164 residue is required for the regulation of DNA damage tolerance (DDT) pathways, pathways that ensure recovery from replication arrest at DNA lesions. |
| Biochem/physiol Actions: | Proliferating cell nuclear antigen (PCNA) is essential for DNA replication during S-phase. It is essential for cellular DNA synthesis. PCNA is required for leading strand synthesis in the SV40 system where it acts as an auxiliary protein for polymerase. |
| Biochem/physiol Actions: | Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Proliferating Cell Nuclear Antigen (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with PCNA-Protein A fusion protein. Proliferating cell nuclear antigen (PCNA, 36 kDa), also known as cyclin, is an auxiliary protein of DNA polymerase. The protein is present in the nucleoplasm of continually cycling cells throughout the cell cycle. |
| General description: | PCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets. |
| Immunogen: | PCNA-Protein A fusion protein |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |