Phospholipase A2 from bovine pancreas
SIGMA/P8913 - lyophilized powder, ≥20 units/mg protein
Synonym: Lecithinase A; PLA2; Phosphatidylcholine 2-acylhydrolase
CAS Number: 9001-84-7
EC Number: 232-637-7
MDL Number: MFCD00131890
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P8913-50MG
| composition | Protein, 5-20% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥20 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00593  |
| Analysis Note: | Protein determined by biuret. |
| Application: | Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels. |
| Biochem/physiol Actions: | Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells. |
| General description: | Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of soybean |
| Packaging: | 10, 50 mg in poly bottle |
| Packaging: | 5 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Contains Tris buffer salts. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |