Platelet-Derived Growth Factor from porcine platelets
SIGMA/P8953 - PDGF, lyophilized powder, suitable for cell culture
Synonym: PDGF
MDL Number: MFCD00166903
Product Type: Chemical
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| assay | ≥97% (SDS-PAGE and N-terminal analysis) |
| biological source | Porcine |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| mol wt | glycoprotein 38 kDa |
| packaging | pkg of 5 μg |
| potency | 39.00 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P20034  |
| Analysis Note: | The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts. |
| Application: | PDGF elicits multifunctional actions with a variety of cells, including mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. |
| Biochem/physiol Actions: | The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid, pH 2.0. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE and N-terminal analysis) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |