Monoclonal Anti-PINCH-1 antibody produced in mouse
SIGMA/P8996 - clone PINCH-C58, purified immunoglobulin, buffered aqueous solution
MDL Number: MFCD06798713
Product Type: Chemical
Immunoblotting
Cell extracts from several cell lines were separated by SDS-PAGE and probed with Monoclonal Anti-PINCH-C58 (2 μg/mL) (Cat. No. P8996). The antibody was developed with Anti-Mouse IgG, biotin (Cat. No. B7151) and ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636) followed by BCIP substrate. (Lane 1) G361; (Lane 2) 293T; (Lane 3) COS7; (Lane 4) MBDK; (Lane 5) MDCK; (Lane 6) ROS; (Lane 7) CHO; (Lane 8) 3T3.
Cell extracts from several cell lines were separated by SDS-PAGE and probed with Monoclonal Anti-PINCH-C58 (2 μg/mL) (Cat. No. P8996). The antibody was developed with Anti-Mouse IgG, biotin (Cat. No. B7151) and ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636) followed by BCIP substrate. (Lane 1) G361; (Lane 2) 293T; (Lane 3) COS7; (Lane 4) MBDK; (Lane 5) MDCK; (Lane 6) ROS; (Lane 7) CHO; (Lane 8) 3T3.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PINCH-C58, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~37 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | hamster, bovine, mouse, monkey, canine, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using total cell extract from 293T cells | |
| UniProt accession no. | P48059  |
| Application: | Monoclonal Anti-PINCH-1 antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • co-immunoprecipitation |
| Biochem/physiol Actions: | The interaction with PINCH proteins (PINCH-1 and 2) is important for localization of integrin linked kinase protein to cell matrix contact sites. It plays a key role in cell−matrix adhesion. PINCH2 is co-expressed and co-localized with PINCH-1/ILK complex. PINCH1 interacts with Wilms tumor 1 protein (WT1) in podocytes. Elevated expression of PINCH1 is observed in human laryngeal carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PINCH-1 (mouse IgG1 isotype) is derived from the hybridoma PINCH-C58 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a peptide of human PINCH1, conjugated to KLH. LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1) or particularly interesting new Cys-His protein 1 (PINCH-1) is widely expressed. It has five LIM domains. It has leucine-rich nuclear export signal (NES) and nuclear localization signal (NLS) at the C-terminus. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence. |
| Immunogen: | peptide corresponding to amino acids 281-299 of human PINCH-1, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |