Anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody produced in rabbit
SIGMA/P8997 - affinity isolated antibody, lyophilized powder
Synonym: Anti-P2Z
MDL Number: MFCD06798528
Product Type: Chemical
Immunocytochemistry Anti-P2X7 Receptor (extracellular)-FITC: Cat. No. P8997: Immunocytochemistry of K562 living cells stained with Anti-P2X7 Receptor (extracellular)-FITC antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 10 μg/test using Jurkat T-cells |
| UniProt accession no. | Q99572  |
| Application: | Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody is used to tag Purinergic Receptor P2X7 (extracellular) for detection and quantitation by fluorescence-based immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Purinergic Receptor P2X7 (extracellular) in ATP-dependent processes such as cell lysis and calcium movement. |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody reacts with rat, human, and mouse purinergic receptor P2X7 (extracellular). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Purinergic Receptor P2X7 is a ionotrophic ATP-gated channel responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. ATP activation of the P2RX7 leads to recruitment of pannexin pores which allow small molecules such as ATP to leak out of cells further activating of purinergic receptors and physiological responses such a spreading cytoplasmic waves of calcium. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm. |
| Immunogen: | synthetic peptide corresponding to residues 136-152 of mouse P2X7 receptor. Sequence homology with human, mouse, and rat is identical. Bovine is 14/17 residues identical. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |