Anti-p53 antibody, Mouse monoclonal
SIGMA/P8999 - clone DO-7, purified from hybridoma cell culture
Synonym: Anti-p53 antibody, Mouse monoclonal; Anti-TRP53; Anti-
MDL Number: MFCD00164709
Product Type: Chemical
Western Blotting JOURNAL CITATION: The FGF-1-specific single-chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis. By: Shi, H., Fu, C., et al. in J Cell Mol Med, 2014. PubMed ID: 25124967 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/jcmm.12371), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.25 μg/mL Monoclonal Anti-p53 Clone: DO-7 (Cat. No. P8999). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. A431 3. RAJI
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DO-7, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~53 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 0.1-0.2 μg/mL using human A431 | |
| UniProt accession no. | P04637  |
| Application: | Monoclonal Anti-p53 antibody produced in mouse has been used in: • immunoblotting • enzyme linked immunosorbent assay (ELISA) • immunocytochemistry • immunohistochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest. |
| Biochem/physiol Actions: | p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |