PNGase F from Elizabethkingia meningoseptica
SIGMA/P9120 - recombinant, expressed in E. coli, set of 100 units nanomolar unit
Synonym: N-Glycanase®; N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase
CAS Number: 83534-39-8
MDL Number: MFCD18839214
Product Type: Chemical
Figure 4. Cleavage site and structural requirements for PNGase F.
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
| conjugate | (N-linked) |
| mol wt | 36 kDa |
| packaging | set of 100 units nanomolar unit |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | ≥10 units/mg protein |
| storage temp. | 2-8°C |
| Application: | PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation • of recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA) • of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins) • of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrPc) post transfection |
| Application: | PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins. |
| Application: | Used to deglycosylate protein. |
| Biochem/physiol Actions: | Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. |
| Biochem/physiol Actions: | PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F. |
| Legal Information: | N-Glycanase is a registered trademark of Agilent Technologies Inc |
| Other Notes: | One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit. |
| Packaging: | Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions |
| Symbol |    GHS05,GHS06,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H317 - H318 - H410 |
| Precautionary statements | P273 - P280 - P305 + P351 + P338 - P312 - P501 |
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-41-43-50/53 |
| Safety Statements | 26-36/37/39-61 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |