Anti-Purinergic Receptor P2X7 (extracellular) antibody produced in rabbit
SIGMA/P9122 - affinity isolated antibody, lyophilized powder
Synonym: Anti-P2Z
MDL Number: MFCD06798527
Product Type: Chemical
Immunohistochemistry Anti-P2X7 Receptor (extracellular): Cat. No. P9122: Immunohistochemical staining of Rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) antibody, (1:100), followed by Mouse Anti-Rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
Immunocytochemistry Anti-P2X7 Receptor (extracellular): Cat. No. P9122: Immunocytochemical staining of intact living Rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) antibody, (1:100) followed by Goat Anti-Rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:200 using rat brain lysate and human cell lines |
| UniProt accession no. | Q99572  |
| Application: | Anti-Purinergic Receptor P2(X7) (extracellular) antibody is suitable for use in immunoblot and western blot (1:200, using rat brain lysate and human cell lines). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | P2X receptors are membrane-bound ion channels that are activated by extracellular ATP. These receptors consist of seven known isoforms ranging from P2X1 to P2X7 . P2X7 modulates several cellular functions such as cell death, cytokine release and mRNA synthesis . Anti-Purinergic Receptor P2(X7) (extracellular) antibody is specific for P2X7 in rats and mice. The antibody also reacts with human K562 (Chronic myelogeneous leukemia); WEHI-231 (Lymphoma, B lymphocyte) and HL-60 (promyelocytic leukemia) cell lines. |
| Immunogen: | Synthetic peptide corresponding to residues 136-152 of mouse P2X7 receptor. This antigenic epitope is identical in humans and rats; whereas in bovines, only 14/17 residues are identical. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, 1% BSA, and 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |