Pyruvate Kinase from rabbit muscle
SIGMA/P9136 - Type III, lyophilized powder, 350-600 units/mg protein
Synonym: ATP:pyruvate 2-
CAS Number: 9001-59-6
EC Number: 232-616-2
MDL Number: MFCD00081946
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9136-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9136-50KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9136-5KU
| biological source | rabbit muscle |
| foreign activity | lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01% |
| form | lyophilized powder |
| mol wt | 237 kDa |
| Quality Level | 200  |
| specific activity | 350-600 units/mg protein |
| storage temp. | −20°C |
| type | Type III |
| Analysis Note: | Protein determined by biuret. |
| Application: | Pyruvate Kinase from rabbit muscle has been used to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) in Pseudomonas putida cells. |
| Application: | Pyruvate kinase has been used in plant spectrophotometric assays to measure ATP hydrolysis . Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency . |
| Biochem/physiol Actions: | Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa. Isoelectric Point: 7.6 Optimal pH: ∼7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP. |
| Biochem/physiol Actions: | Pyruvate kinase (PK) catalyzes an important process of transferring phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP). This reaction results in the conversion of PEP to pyruvate and adenosine triphosphate (ATP). Pyruvate kinase plays a major role in glycolysis and gluconeogenesis. High levels of pyruvate kinase M2 (PKM2) inhibits cell proliferation and tumor growth. Deficiency of PK in red blood cells (RBC) leads to non-spherocytic hemolytic anaemia. In human erythrocytes, deficiency of PK induces a protective effect against Plasmodium falciparum. |
| General description: | Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells. |
| Other Notes: | One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C. |
| Packaging: | 1000, 5000, 25000, 50000 units in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 350-600 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.40 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |